Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸ 10 µl 127 315 ₸

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Anti-MVP antibody [EPR13226(B)] (ab177145)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13226(B)] to MVP
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-MVP antibody [EPR13226(B)]
See all MVP primary antibodies
Description

Rabbit monoclonal [EPR13226(B)] to MVP
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human MVP aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q14764
Positive control
- Human spleen, Calu3 and A549 lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13226(B)
Isotype

IgG
Research areas

Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)

All lanes : Anti-MVP antibody [EPR13226(B)] (ab177145) at 1/1000 dilution

Lane 1 : Wild-type HeLa lysate
Lane 2 : MVP knockout HeLa lysate
Lane 3 : A549 lysate
Lane 4 : MOLT-4 lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 99 kDa

Lanes 1-4: Merged signal (red and green). Green - ab177145 observed at 99 kDa. Red - loading control ab8245 observed at 37 kDa.

ab177145 Anti-MVP antibody [EPR13226(B)] was shown to specifically react with MVP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264817 (knockout cell lysate ab257544) was used. Wild-type and MVP knockout samples were subjected to SDS-PAGE. ab177145 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)

All lanes : Anti-MVP antibody [EPR13226(B)] (ab177145) at 1/1000 dilution

Lane 1 : Human spleen lysate
Lane 2 : Calu3 lysate
Lane 3 : A549 lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 99 kDa
Anti-MVP antibody [EPR13226(B)] (ab177145)

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