Anti-MVP antibody - N-terminal (ab226464)
Images
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Western blot - Anti-MVP antibody - N-terminal (ab226464)All lanes : Anti-MVP antibody - N-terminal (ab226464) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 99 kDa
Exposure time: 3 minutesCells prepared using NETN lysis buffer.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody - N-terminal (ab226464)Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for MVP using ab226464 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
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Immunoprecipitation - Anti-MVP antibody - N-terminal (ab226464)MVP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226464 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226464 at 1 µg/ml.
Lane 1: ab226464 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Cells prepared using NETN lysis buffer.
