Anti-KPNA4 antibody (ab6039)
Key features and details
- Goat polyclonal to KPNA4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-KPNA4 antibody
See all KPNA4 primary antibodies -
Description
Goat polyclonal to KPNA4 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog
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Immunogen
Synthetic peptide corresponding to Human KPNA4 aa 500-600 (C terminal).
Database link: NP_002259 -
Positive control
- WB: Human ovary lysate. Mouse ovary and testis lysate. Rat testis lysate.
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General notes
GenBank Accession Number – NP_002259.The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KPNA4 antibody (ab6039)All lanes : Anti-KPNA4 antibody (ab6039) at 1 µg/ml
Lane 1 : Human testis
Lane 2 : Human ovary
Lane 3 : Mouse testis
Lane 4 : Rat testis
Lane 5 : Rat ovary
Lysates/proteins at 35 µg per lane.
Predicted band size: 58 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody (ab6039) Image is courtesy of an anonymous AbReview.Immunohistochemical analysis of 10% buffered formalin-fixed paraffin-embedded murine nephric tissue sections, labelling KPNA4 with ab6039 at a dilution of 1/100 incubated for 12 hours at 4°C. Heat mediated antigen retrival was performed through 10mM citrate buffer at pH 6.0. Blocking was with 5% serum incubated for 1 hour at 21°C. Secondary was a polyclonal Donkey anti-Goat Alexa Fluor® 647 conjugate at 1/200. Counterstain is DAPI against nuclear DNA. The Negative control is the same as above, but in the abscence of the primary ab6039.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody (ab6039)ab6039 (3µg/ml) staining Human Testis by IHC-P. Microwaved antigen retrieval with citrate buffer (pH 6), HRP-staining.
