Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MTAP antibody [EPR22574-104] - BSA and Azide free (ab256358)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22574-104] to MTAP - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MTAP antibody [EPR22574-104] - BSA and Azide free
    See all MTAP primary antibodies

  • Description

    Rabbit monoclonal [EPR22574-104] to MTAP - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    IHC-P is recommended for human only.

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: C6, NIH/3T3, HT-29 and MDA-MD-435S whole cell lysates. IP: HT-29 whole cell lysate. IHC-P: Human breast, bladder cancer and breast cancer tissue.

  • General notes

    Ab256358 is the carrier-free version of ab252934. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256358 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-MTAP antibody [EPR22574-104] - BSA and Azide free (ab256358)
    Immunoprecipitation - Anti-MTAP antibody [EPR22574-104] - BSA and Azide free (ab256358)

    MTAP was immunoprecipitated from 0.35  mg of HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate with ab252934 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab252934 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.


    Lane 1: HT-29 whole cell lysate 10 μg (Input).
    Lane 2: ab252934 IP in HT-29 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252934 in HT-29 whole cell lysate.


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252934).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22574-104] - BSA and Azide free (ab256358)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22574-104] - BSA and Azide free (ab256358)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling MTAP with ab252934 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in stroma cells and no staining in tumor cells of human breast cancer (PMID:26751376) is observed. Counter stained with hematoxylin.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.


    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252934).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22574-104] - BSA and Azide free (ab256358)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22574-104] - BSA and Azide free (ab256358)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling MTAP with ab252934 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human breast cancer (PMID:26751376,18712977) is observed. Counter stained with hematoxylin.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.


    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252934).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22574-104] - BSA and Azide free (ab256358)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22574-104] - BSA and Azide free (ab256358)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling MTAP with ab252934 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in stroma cells and no staining in tumor cells of human bladder cancer (PMID: 27270441) is observed. Counter stained with hematoxylin.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.


    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252934).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22574-104] - BSA and Azide free (ab256358)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22574-104] - BSA and Azide free (ab256358)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling MTAP with ab252934 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human breast (PMID:26751376, 18712977) is observed. Counter stained with hematoxylin.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.


    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252934).
  • Anti-MTAP antibody [EPR22574-104] - BSA and Azide free (ab256358)
    Anti-MTAP antibody [EPR22574-104] - BSA and Azide free (ab256358)

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