Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 09, 2021

Anti-MTAP antibody [EPR22574-104] (ab252934)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22574-104] to MTAP
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-MTAP antibody [EPR22574-104]
See all MTAP primary antibodies
Description

Rabbit monoclonal [EPR22574-104] to MTAP
Host species

Rabbit
Specificity

IHC-P is recommended for human only.

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: C6, NIH/3T3, HT-29 and MDA-MD-435S whole cell lysates. IP: HT-29 whole cell lysate. IHC-P: Human breast, bladder cancer and breast cancer tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22574-104
Isotype

IgG
Research areas

Immunoprecipitation - Anti-MTAP antibody [EPR22574-104] (ab252934)

MTAP was immunoprecipitated from 0.35 mg of HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate with ab252934 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab252934 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1: HT-29 whole cell lysate 10 μg (Input).
Lane 2: ab252934 IP in HT-29 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252934 in HT-29 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22574-104] (ab252934)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling MTAP with ab252934 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in stroma cells and no staining in tumor cells of human breast cancer (PMID:26751376) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-MTAP antibody [EPR22574-104] (ab252934)

All lanes : Anti-MTAP antibody [EPR22574-104] (ab252934) at 1/1000 dilution

Lane 1 : C6 (rat glial tumor glial cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 31 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-MTAP antibody [EPR22574-104] (ab252934)

All lanes : Anti-MTAP antibody [EPR22574-104] (ab252934) at 1/1000 dilution

Lane 1 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : MDA-MB-235S (human ductal carcinoma cell line) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 31 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.

Negative control: A549 (PMID: 17548352).

The expression molecular weight observed is consistent with what has been described in the literature (PMID:15492751).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22574-104] (ab252934)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling MTAP with ab252934 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human breast cancer (PMID:26751376,18712977) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22574-104] (ab252934)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling MTAP with ab252934 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in stroma cells and no staining in tumor cells of human bladder cancer (PMID: 27270441) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22574-104] (ab252934)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling MTAP with ab252934 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human breast (PMID:26751376, 18712977) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-MTAP antibody [EPR22574-104] (ab252934)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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