Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATTY antibody (ab91450)

Key features and details

  • Rabbit polyclonal to ATTY
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-ATTY antibody
    See all ATTY primary antibodies

  • Description

    Rabbit polyclonal to ATTY

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Zebrafish

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: NIH3T3; HepG2; PC12; A20; Caco2; SKNBE; MCF7; K562; Raji; A431.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab91450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has no transaminase activity towards phenylalanine.

  • Pathway

    Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 2/6.

  • Involvement in disease

    Defects in TAT are the cause of tyrosinemia type 2 (TYRO2) [MIM:276600]; also known as Richner-Hanhart syndrome. TYRO2 is an inborn error of metabolism characterized by elevations of tyrosine in the blood and urine, and oculocutaneous manifestations. Typical features include palmoplantar keratosis, painful corneal ulcers, and mental retardation.

  • Sequence similarities

    Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ATTY_HUMAN antibody
    • L-tyrosine:2-oxoglutarate aminotransferase antibody
    • TAT antibody
    • Tyrosine aminotransferase antibody
    • Tyrosine aminotransferase, cytosolic antibody
    see all

Images

  • Western blot - Anti-ATTY antibody (ab91450)
    Western blot - Anti-ATTY antibody (ab91450)
    All lanes : Anti-ATTY antibody (ab91450) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : A20 whole cell lysate (ab7180)
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 8 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 9 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 10 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa
    Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 16 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-ATTY antibody (ab91450)
    Immunocytochemistry/ Immunofluorescence - Anti-ATTY antibody (ab91450)
    ICC/IF image of ab91450 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91450, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References (0)

Publishing research using ab91450? Please let us know so that we can cite the reference in this datasheet.

ab91450 has not yet been referenced specifically in any publications.

Images

  • Western blot - Anti-ATTY antibody (ab91450)
    Western blot - Anti-ATTY antibody (ab91450)
    All lanes : Anti-ATTY antibody (ab91450) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : A20 whole cell lysate (ab7180)
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 8 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 9 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 10 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa
    Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 16 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-ATTY antibody (ab91450)
    Immunocytochemistry/ Immunofluorescence - Anti-ATTY antibody (ab91450)
    ICC/IF image of ab91450 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91450, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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