Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CRM1 antibody [EPR14124] - BSA and Azide free (ab251055)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14124] to CRM1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CRM1 antibody [EPR14124] - BSA and Azide free
    See all CRM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14124] to CRM1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251055 is the carrier-free version of ab191081.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CRM1 antibody [EPR14124] - BSA and Azide free (ab251055)
    Western blot - Anti-CRM1 antibody [EPR14124] - BSA and Azide free (ab251055)
    All lanes : Anti-CRM1 antibody [EPR14124] - C-terminal (ab191081) at 1/5000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 123 kDa



    This data was developed using ab191081, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRM1 antibody [EPR14124] - BSA and Azide free (ab251055)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRM1 antibody [EPR14124] - BSA and Azide free (ab251055)
    This data was developed using ab191081, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human bladder transitional cell carcinoma tissue labeling CRM1 with ab191081 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-CRM1 antibody [EPR14124] - BSA and Azide free (ab251055)
    Immunocytochemistry - Anti-CRM1 antibody [EPR14124] - BSA and Azide free (ab251055)
    This data was developed using ab191081, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed; 0.1% tritonX-100-permeabilized) labeling CRM1 with ab191081 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
  • Flow Cytometry - Anti-CRM1 antibody [EPR14124] - BSA and Azide free (ab251055)
    Flow Cytometry - Anti-CRM1 antibody [EPR14124] - BSA and Azide free (ab251055)
    This data was developed using ab191081, the same antibody clone in a different buffer formulation.Flow cytometric analysis of K562 cells (paraformaldehyde-fixed, 2%) labeling CRM1 with ab191081 at 1/160 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
  • Western blot - Anti-CRM1 antibody [EPR14124] - BSA and Azide free (ab251055)
    Western blot - Anti-CRM1 antibody [EPR14124] - BSA and Azide free (ab251055)
    All lanes : Anti-CRM1 antibody [EPR14124] - C-terminal (ab191081) at 1/20000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Ramos cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 123 kDa



    This data was developed using ab191081, the same antibody clone in a different buffer formulation.

     

  • Anti-CRM1 antibody [EPR14124] - BSA and Azide free (ab251055)
    Anti-CRM1 antibody [EPR14124] - BSA and Azide free (ab251055)

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