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Signal Transduction Second Messenger Nucleotide Messenger GTP

Anti-RAB45 antibody [EPR16349] - BSA and Azide free (ab251195)

Anti-RAB45 antibody [EPR16349] - BSA and Azide free (ab251195)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16349] to RAB45 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-RAB45 antibody [EPR16349] - BSA and Azide free
    See all RAB45 primary antibodies
  • Description

    Rabbit monoclonal [EPR16349] to RAB45 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251195 is the carrier-free version of ab194827.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16349
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • GTP
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Images

  • Immunocytochemistry - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (ab251195)
    Immunocytochemistry - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (ab251195)

    This data was developed using ab194827, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of HeLa cells labeling RAB45 with ab194827 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on HeLa cell line was observed.
  • Western blot - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (ab251195)
    Western blot - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (ab251195)
    All lanes : Anti-RAB45 antibody [EPR16349] (ab194827) at 1/10000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : Rat heart tissue lysate
    Lane 6 : C6 cell lysate
    Lane 7 : RAW 264.7 cell lysate
    Lane 8 : PC-12 cell lysate
    Lane 9 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 83 kDa



    This data was developed using ab194827, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (ab251195)
    Western blot - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (ab251195)
    Anti-RAB45 antibody [EPR16349] (ab194827) at 1/10000 dilution + K562 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 83 kDa



    This data was developed using ab194827, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (ab251195)
    Western blot - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (ab251195)
    All lanes : Anti-RAB45 antibody [EPR16349] (ab194827) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 83 kDa



    This data was developed using ab194827, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (ab251195)
    Flow Cytometry - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (ab251195)
    This data was developed using ab194827, the same antibody clone in a different buffer formulation.Flow cytometry analysis of K562 cells labeling RAB45 using ab194827 at 1/50 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody: Blue. Rabbit monoclonal IgG was used as isotype control (Black).
  • Anti-RAB45 antibody [EPR16349] - BSA and Azide free (ab251195)
    Anti-RAB45 antibody [EPR16349] - BSA and Azide free (ab251195)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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