Anti-RAB45 antibody [EPR16349] - BSA and Azide free (ab251195)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16349] to RAB45 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-RAB45 antibody [EPR16349] - BSA and Azide free
See all RAB45 primary antibodies -
Description
Rabbit monoclonal [EPR16349] to RAB45 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251195 is the carrier-free version of ab194827.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16349 -
Isotype
IgG -
Research areas
Images
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This data was developed using ab194827, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of HeLa cells labeling RAB45 with ab194827 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on HeLa cell line was observed. -
All lanes : Anti-RAB45 antibody [EPR16349] (ab194827) at 1/10000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat heart tissue lysate
Lane 6 : C6 cell lysate
Lane 7 : RAW 264.7 cell lysate
Lane 8 : PC-12 cell lysate
Lane 9 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 83 kDaThis data was developed using ab194827, the same antibody clone in a different buffer formulation.
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All lanes : Anti-RAB45 antibody [EPR16349] (ab194827) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Human testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 83 kDaThis data was developed using ab194827, the same antibody clone in a different buffer formulation.
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This data was developed using ab194827, the same antibody clone in a different buffer formulation.Flow cytometry analysis of K562 cells labeling RAB45 using ab194827 at 1/50 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody: Blue. Rabbit monoclonal IgG was used as isotype control (Black).
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