Anti-LYAR antibody [EPR14352] - BSA and Azide free (ab250474)
![Anti-LYAR antibody [EPR14352] - BSA and Azide free (ab250474)](https://www.abcam.com/ps/products/250/ab250474/Images/ab250474-405887-anti-lyar-antibody-epr14352-bsa-and-azide-free-western-blot.jpg "Anti-LYAR antibody [EPR14352] - BSA and Azide free (ab250474)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14352] to LYAR - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-LYAR antibody [EPR14352] - BSA and Azide free
See all LYAR primary antibodies -
Description
Rabbit monoclonal [EPR14352] to LYAR - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250474 is the carrier-free version of ab181261. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250474 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR14352 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LYAR antibody [EPR14352] - BSA and Azide free (ab250474)All lanes : Anti-LYAR antibody [EPR14352] (ab181261) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution
Predicted band size: 44 kDaThis data was developed using ab181261, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-LYAR antibody [EPR14352] - BSA and Azide free (ab250474)](https://www.abcam.com/ps/products/250/ab250474/Images/ab250474-402770-anti-lyar-antibody-epr14352-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-LYAR antibody [EPR14352] - BSA and Azide free (ab250474) -
![Immunocytochemistry - Anti-LYAR antibody [EPR14352] - BSA and Azide free (ab250474)](https://www.abcam.com/ps/products/250/ab250474/Images/ab250474-1-anti-lyar-antibody-epr14352-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-LYAR antibody [EPR14352] - BSA and Azide free (ab250474)This data was developed using ab181261, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling LYAR with ab181261 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution and counter-stained with DAPI (blue). -
![Anti-LYAR antibody [EPR14352] - BSA and Azide free (ab250474)](https://www.abcam.com/ps/products/250/ab250474/Images/ab250474-4-benefits-of-recombinant-antibodies.png)
Anti-LYAR antibody [EPR14352] - BSA and Azide free (ab250474)