Anti-CRF antibody [EPR19154] - BSA and Azide free (ab250741)
![Anti-CRF antibody [EPR19154] - BSA and Azide free (ab250741)](https://www.abcam.com/ps/products/250/ab250741/Images/ab250741-403513-anti-crf-antibody-epr19154-bsa-and-azide-free-western-blot.jpg "Anti-CRF antibody [EPR19154] - BSA and Azide free (ab250741)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19154] to CRF - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CRF antibody [EPR19154] - BSA and Azide free
See all CRF primary antibodies -
Description
Rabbit monoclonal [EPR19154] to CRF - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250741 is the carrier-free version of ab184238 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250741 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Corticotropin Releasing Factor , Corticotropin Releasing Factor
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19154 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CRF antibody [EPR19154] - BSA and Azide free (ab250741)Anti-CRF antibody [EPR19154] (ab184238) at 1/1000 dilution + Human CRF fragment recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 1 secondThis data was developed using ab184238, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human CRF fragment recombinant protein contains aa20-185 with a His-Tag®. This recombinant human fragment protein was made in-house.
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![Western blot - Anti-CRF antibody [EPR19154] - BSA and Azide free (ab250741)](https://www.abcam.com/ps/products/250/ab250741/Images/ab250741-398218-anti-crf-antibody-epr19154-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CRF antibody [EPR19154] - BSA and Azide free (ab250741)All lanes : Anti-CRF antibody [EPR19154] (ab184238) at 1/5000 dilution
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse hypothalamus lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat hypothalamus lysate
Lane 6 : Mouse pancreas lysate
Lane 7 : Mouse skin lysate
Lane 8 : Rat skin lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab184238, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-CRF antibody [EPR19154] - BSA and Azide free (ab250741)](https://www.abcam.com/ps/products/250/ab250741/Images/ab250741-3-benefits-of-recombinant-antibodies.png)
Anti-CRF antibody [EPR19154] - BSA and Azide free (ab250741)