Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human Oxytocin Prepropeptide ELISA Kit (ab242248)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 10 pg/ml
  • Range: 125 pg/ml - 8000 pg/ml
  • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Oxytocin Prepropeptide ELISA Kit
    See all Oxytocin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    serum 8 3.2%

  • Sample type

    Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    10 pg/ml

  • Range

    125 pg/ml - 8000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 90 84% - 100%
    Hep Plasma 104 94% - 113%
    EDTA Plasma 104 95% - 111%
    Cit plasma 92 83% - 99%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Oxytocin Prepropeptide ELISA Kit (ab242248) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Oxytocin Prepropeptide protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Oxytocin Prepropeptide with 10 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human Oxytocin ELISA Kit (ab242248)
    Other - Human Oxytocin ELISA Kit (ab242248)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human Oxytocin standard curve in Sample Diluent NS.
    Example of human Oxytocin standard curve in Sample Diluent NS.
    The Oxytocin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
  • Interpolated concentrations of native Oxytocin in human serum and plasma samples.
    Interpolated concentrations of native Oxytocin in human serum and plasma samples.
    The concentrations of Oxytocin were measured in duplicates, interpolated from the Oxytocin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 50%, plasma (EDTA) 25%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Oxytocin concentration was determined to be 23,301 pg/mL in serum, 13,420 pg/mL in plasma (citrate), 21,214 pg/mL in plasma (EDTA), and 20843 pg/mL in plasma (Heparin).
  • Sandwich ELISA - Human Oxytocin Prepropeptide ELISA Kit (ab242248)
    Sandwich ELISA - Human Oxytocin Prepropeptide ELISA Kit (ab242248)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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