Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HE4 antibody [EPR16658] (ab200828)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16658] to HE4
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-HE4 antibody [EPR16658]
    See all HE4 primary antibodies

  • Description

    Rabbit monoclonal [EPR16658] to HE4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human ovary cancer and NIH:OVCAR-3 whole cell lysate. IHC-P: Human ovarian carcinoma. IP: OVCAR-3 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16658

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-HE4 antibody [EPR16658] (ab200828)
    Western blot - Anti-HE4 antibody [EPR16658] (ab200828)
    All lanes : Anti-HE4 antibody [EPR16658] (ab200828) at 1/1000 dilution

    Lane 1 : Human ovary cancer whole cell lysate
    Lane 2 : NIH:OVCAR-3 (Human ovary adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 13 kDa
    Observed band size: 17-25 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/dilution buffer: 5% NFDM/TBST.

     

    The expression profile observed is consistent with what has been described in the literature PMID:15781627

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HE4 antibody [EPR16658] (ab200828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HE4 antibody [EPR16658] (ab200828)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma labeling HE4 with ab200828 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on Human ovarian carcinoma tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-HE4 antibody [EPR16658] (ab200828)
    Immunoprecipitation - Anti-HE4 antibody [EPR16658] (ab200828)

    HE4 was immunoprecipitated from 1mg of NIH:OVCAR-3 (Human ovary adenocarcinoma ) whole cell lysate with ab200828 at 1/120 dilution.

    Western blot was performed from the immunoprecipitate using ab200828 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: NIH:OVCAR-3 (Human ovary adenocarcinoma) whole cell lysate 10µg.

    Lane 2: NIH:OVCAR-3 (Human ovary adenocarcinoma) whole cell lysate following IP.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200828 in NIH:OVCAR-3 (human ovary adenocarcinoma) whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST. 10 second exposure.

  • Anti-HE4 antibody [EPR16658] (ab200828)
    Anti-HE4 antibody [EPR16658] (ab200828)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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