Secretory Phospholipase A2 Assay Kit (ab133089)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Plasma
Overview
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Product name
Secretory Phospholipase A2 Assay Kit -
Detection method
Colorimetric -
Sample type
Plasma -
Assay type
Enzyme activity (quantitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Secretory Phospholipase A2 Assay Kit (ab133089) provides an accurate and convenient method for measurement of secretory PLA2 (sPLA2) activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s (e.g., bee and cobra venoms, pancreatic, etc.) with the exception of cytosolic PLA2. Upon hydrolysis of the thio ester bond at the sn-2 position by PLA2, free thiols are detected using DTNB (5,5’-dithio-bis-(2-nitrobenzoic acid)).
If you want to detect cytosolic PLA2, we recommend using Cytosolic Phospholipase A2 Assay Kit (ab133090).
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 96 tests 96-Well Plate (Colorimetric Assay) 1 x 96 tests 96-Well Plate Cover 1 unit Bee venom PLA2 Control 1 vial sPLA2 Assay Buffer (10X) 1 vial sPLA2 Diheptanoyl Thio-PC (Substrate) 2 vials sPLA2 DTNB 4 vials -
Research areas
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Function
Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. -
Sequence similarities
Belongs to the phospholipase A2 family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- EC 3.1.1.4
- GIIC sPLA2
- Group IIA phospholipase A2
see all
Images
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Functional Studies - Secretory Phospholipase A2 Assay Kit (ab133089)Absorbance times for bee venom, snake venom, human synovial, and bovine pancreas PLA2s.