Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19497] to Trypsin - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-Trypsin antibody [EPR19497] - BSA and Azide free
    See all Trypsin primary antibodies

  • Description

    Rabbit monoclonal [EPR19497] to Trypsin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251325 is the carrier-free version of ab200996.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
    Western blot - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
    All lanes : Anti-PRSS1 antibody [EPR19497] (ab200996) at 1/1000 dilution

    Lane 1 : Human PRSS1 fragment recombinant protein
    Lane 2 : Human TRY3 full length recombinant protein
    Lane 3 : Human TRY6 full length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa



    This data was developed using ab200996, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 1 second; Lanes 2-3: 3 minutes.

    Human PRSS1 fragment recombinant protein contains aa24-122 with a His-Tag®. Human TRY3 full length recombinant protein contains aa1-304 with a His-Tag®. Human TRY6 full length recombinant protein contains aa1-247 with a His-Tag®. These three recombinant proteins were made in-house.

  • Western blot - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
    Western blot - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
    Anti-PRSS1 antibody [EPR19497] (ab200996) at 1/1000 dilution + Human TRY2 full length recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa


    Exposure time: 3 minutes


    This data was developed using ab200996, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human TRY2 full length recombinant protein contains aa1-247 with a GST-Tag. It was made in-house.

  • Western blot - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
    Western blot - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
    All lanes : Anti-PRSS1 antibody [EPR19497] (ab200996) at 1/5000 dilution

    Lane 1 : Human pancreas lysate
    Lane 2 : Human fetal liver tissue lysate
    Lane 3 : Human fetal heart tissue lysate
    Lane 4 : Human fetal kidney tissue lysate
    Lane 5 : Human fetal spleen tissue lysate
    Lane 6 : Human fetal lung tissue lysate
    Lane 7 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate
    Lane 8 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
    Lane 9 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa


    Exposure time: 3 minutes


    This data was developed using ab200996, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Trypsin 1, encoded by the PRSS1 gene, is synthesized in the pancreas. The expression profile observed is consistent with what has been described in the literatures (PMID: 3011602, 7945238, 24722290).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
    This data was developed using ab200996, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PRSS1 with ab200996 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on acinar cells Human pancreas is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
    This data was developed using ab200996, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRSS1 with ab200996 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human kidney. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
    Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)

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