Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GGT1/GGT antibody [EPR5288] (ab109427)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5288] to GGT1/GGT
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-GGT1/GGT antibody [EPR5288]
    See all GGT1/GGT primary antibodies

  • Description

    Rabbit monoclonal [EPR5288] to GGT1/GGT

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver and human fetal kidney tissue lysates and HepG2 and A549 cell lysates. IHC-P: Human kidney and stomach tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as GGT1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-GGT1/GGT antibody [EPR5288] (ab109427)
    Western blot - Anti-GGT1/GGT antibody [EPR5288] (ab109427)
    All lanes : Anti-GGT1/GGT antibody [EPR5288] (ab109427) at 1/1000 dilution (purified)

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : Human fetal kidney tissue lysate
    Lane 4 : A549 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 61 kDa
    Observed band size: 50-60 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (ab109427)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (ab109427)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling GGT1/GGT with purified ab109427 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-GGT1/GGT antibody [EPR5288] (ab109427)
    Western blot - Anti-GGT1/GGT antibody [EPR5288] (ab109427)
    All lanes : Anti-GGT1/GGT antibody [EPR5288] (ab109427) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal liver lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (ab109427)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (ab109427)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GGT1/GGT with unpurified ab109427 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-GGT1/GGT antibody [EPR5288] (ab109427)
    Anti-GGT1/GGT antibody [EPR5288] (ab109427)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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