Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PSMA antibody [EP3253] (ab76104)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3253] to PSMA
  • Suitable for: ICC/IF, WB, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-PSMA antibody [EP3253]
    See all PSMA primary antibodies

  • Description

    Rabbit monoclonal [EP3253] to PSMA

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PSMA (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: LNCaP cell lysate. IP: LNCaP cell lysate. Flow Cyt: LNCaP cells. ICC/IF: LNCaP cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-PSMA antibody [EP3253] (ab76104)
    Western blot - Anti-PSMA antibody [EP3253] (ab76104)
    Anti-PSMA antibody [EP3253] (ab76104) at 1/1000 dilution (Purified) + LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 84 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-PSMA antibody [EP3253] (ab76104)
    Immunocytochemistry/ Immunofluorescence - Anti-PSMA antibody [EP3253] (ab76104)
    Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostatecarcinoma epithelial cell) cells labeling PSMA with Purified ab76104 at 1/50 (3.36 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunoprecipitation - Anti-PSMA antibody [EP3253] (ab76104)
    Immunoprecipitation - Anti-PSMA antibody [EP3253] (ab76104)

    Purified ab76104 at 1/20 dilution (1ug) immunoprecipitating PSMA in LNCaP whole cell lysate.
    Lane 1 (input): LNCaP (Human prostatecarcinoma epithelial cell) whole cell lysate (10µg)
    Lane 2 (+): ab76104 + LNCaP whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76104 in LNCaP whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 110 kDa

  • Flow Cytometry - Anti-PSMA antibody [EP3253] (ab76104)
    Flow Cytometry - Anti-PSMA antibody [EP3253] (ab76104)
    Flow Cytometry analysis of LNCaP (Human prostatecarcinoma epithelial cell) cells labeling PSMA with Purified ab76104 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Anti-PSMA antibody [EP3253] (ab76104)
    Anti-PSMA antibody [EP3253] (ab76104)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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