Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP3253] to PSMA
Suitable for: ICC/IF, WB, IP, Flow Cyt
Reacts with: Human

Product name

Anti-PSMA antibody [EP3253]
See all PSMA primary antibodies
Description

Rabbit monoclonal [EP3253] to PSMA
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human PSMA (N terminal). The exact sequence is proprietary.
Positive control
- WB: LNCaP cell lysate. IP: LNCaP cell lysate. Flow Cyt: LNCaP cells. ICC/IF: LNCaP cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP3253
Isotype

IgG
Research areas

Western blot - Anti-PSMA antibody [EP3253] (ab76104)

Anti-PSMA antibody [EP3253] (ab76104) at 1/1000 dilution (Purified) + LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 84 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

Blocking/Diluting buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-PSMA antibody [EP3253] (ab76104)

Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostatecarcinoma epithelial cell) cells labeling PSMA with Purified ab76104 at 1/50 (3.36 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunoprecipitation - Anti-PSMA antibody [EP3253] (ab76104)

Purified ab76104 at 1/20 dilution (1ug) immunoprecipitating PSMA in LNCaP whole cell lysate.
Lane 1 (input): LNCaP (Human prostatecarcinoma epithelial cell) whole cell lysate (10µg)
Lane 2 (+): ab76104 + LNCaP whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76104 in LNCaP whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 110 kDa
Flow Cytometry - Anti-PSMA antibody [EP3253] (ab76104)

Flow Cytometry analysis of LNCaP (Human prostatecarcinoma epithelial cell) cells labeling PSMA with Purified ab76104 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Anti-PSMA antibody [EP3253] (ab76104)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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