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Immunology Innate Immunity Macrophage / Inflamm.

Anti-NLRP3 antibody (ab214185)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-NLRP3 antibody (ab214185)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to NLRP3
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-NLRP3 antibody
    See all NLRP3 primary antibodies
  • Description

    Rabbit polyclonal to NLRP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NLRP3 aa 1-100 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q96P20

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human lung carcinoma tissue. Mouse intestine and embryo tissue. Rat spinal cord and kidney tissue. WB: Raji, SiHa and MDA-MB-231 cell lysate, mouse spleen and thymus tissue lysate.
  • General notes

    The level of NLRP3 expression can be elevated when cells are treated with LPS, as described in the literature (PMID: 22569257). 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Cancer
    • Growth factors
    • TNF
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)

    Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse intestine tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining.

  • Western blot - Anti-NLRP3 antibody (ab214185)
    Western blot - Anti-NLRP3 antibody (ab214185)
    All lanes : Anti-NLRP3 antibody (ab214185) at 1/300 dilution

    Lane 1 : Raji (human Burkitt's lymphoma cell line) cell lysate
    Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line/0 cell lysate

    Secondary
    All lanes : HRP conjugated goat anti-rabbit IgG at 1/5000 dilution
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)

    Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse embryo tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining.

  • Western blot - Anti-NLRP3 antibody (ab214185)
    Western blot - Anti-NLRP3 antibody (ab214185)
    All lanes : Anti-NLRP3 antibody (ab214185) at 1/1000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Mouse thymus tissue lysate

    Secondary
    All lanes : Conjugated secondary antibody at 1/20000 dilution
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)

    Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human lung carcinoma tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)

    Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse intestine tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)

    Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat kidney tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining.

  • Western blot - Anti-NLRP3 antibody (ab214185)
    Western blot - Anti-NLRP3 antibody (ab214185)
    Anti-NLRP3 antibody (ab214185) at 1/300 dilution + SiHa (Human cervical carcinoma cell line) cell lysate

    Secondary
    HRP-conjugated goat anti-rabbit IgG

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-NLRP3 antibody (ab214185)

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    Recombinant Human NLRP3 protein (Tagged) (ab255792)

    Applications: SDS-PAGE

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    Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (ab264468)

    Applications: ELISA, Flow Cyt, IP, WB

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    Anti-NLRP3 antibody [EPR23094-1] (ab263899)

    Applications: ELISA, Flow Cyt, IP, WB

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    Anti-NLRP3 antibody (ab4207)

    Applications: Flow Cyt, ICC/IF

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