Alpha-L-Fucosidase (FUCA1) Assay Kit (Colorimetric) (ab273316)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Saliva, Serum
Overview
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Product name
Alpha-L-Fucosidase (FUCA1) Assay Kit (Colorimetric)
See all FUCA1 kits -
Detection method
Colorimetric -
Sample type
Saliva, Serum -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Alpha-L-Fucosidase (FUCA1) Assay Kit (Colorimetric) (ab273316) provides a simple, sensitive and high-throughput adaptable approach to detect physiological concentrations of this glycosidase in a variety of biological samples.
In this assay, Alpha-L-Fucosidase uses a synthetic chloro p-nitrophenol derivative (R-pNP) as an α-L-Fucosidase substrate and releases a chlorinated pNP derivative which can be measured kinetically under acidic conditions (OD 405 nm). The assay involves a one-step simple reaction, with minimal sample preparation and does not need a stop solution to complete the reaction.
The assay can detect as low as 20 µU of Alpha-L-Fucosidase activity in a variety of samples.
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Tested applications
Suitable for: Othermore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests DTT (1 M) 1 x 0.25ml FUCA1 Assay Buffer 1 x 7ml FUCA1 Positive Control 1 x 20µl FUCA1 Substrate 1 x 6ml pNP Standard (10 mM) 1 x 0.75ml -
Research areas
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Function
Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. -
Involvement in disease
Defects in FUCA1 are the cause of fucosidosis (FUCA1D) [MIM:230000]. FUCA1D is an autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas. -
Sequence similarities
Belongs to the glycosyl hydrolase 29 family. -
Cellular localization
Lysosome. - Information by UniProt
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Alternative names
- Alpha L fucosidase I
- Alpha L fucoside fucohydrolase
- Alpha-L-fucosidase 1
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Images
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Example data
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Example dataEstimation of FUCA1 rates using the enzyme as a positive control.
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Example dataEstimation of FUCA1 in human serum. Fifty microliters of each undiluted sample was assayed following kit protocols. Fucosidase Activity in Pooled Human serum (in U/L): A: 24.3 ± 2.6, B: 20.3 ± 0.25, C: 26.8 ± 3.7, Average of three samples: 23.8 ± 3.3.
