Anti-FN3K antibody (ab232766)
Key features and details
- Rabbit polyclonal to FN3K
- Suitable for: WB, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-FN3K antibody
See all FN3K primary antibodies -
Description
Rabbit polyclonal to FN3K -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant full length protein (His-tag) aa 1 to the C-terminus. Expressed in E.coli. N-terminal tag.
Database link: Q9H479 -
Positive control
- WB: Recombinant human FN3K protein; HeLa and HEK-293T cell lysates; Pig heart and brain lysates. ICC/IF: HeLa cells.
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-FN3K antibody (ab232766)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FN3K (green) using ab232766 at 20 μg/ml in ICC/IF. FITC-labeled secondary antibody.
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Western blot - Anti-FN3K antibody (ab232766)All lanes : Anti-FN3K antibody (ab232766) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Pig heart lysate
Lane 4 : Pig brain lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 35 kDa
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Western blot - Anti-FN3K antibody (ab232766)Anti-FN3K antibody (ab232766) at 2 µg/ml + Recombinant human FN3K protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 35 kDa
