Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Arg2 antibody [EPR22626-38] (ab264066)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22626-38] to Arg2
Suitable for: IHC-P, WB, IHC-Fr, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Arg2 antibody [EPR22626-38]
See all Arg2 primary antibodies
Description

Rabbit monoclonal [EPR22626-38] to Arg2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Mouse Rat Human
IP	Mouse
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse kidney, Human kidney, Rat kidney, Rat small intestine and Mouse small intestine lysates. IHC-P: Mouse small intestine, Mouse kidney, Rat kidney, Rat colon and Human cerebrum tissues. IP: Mouse kidney lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22626-38
Isotype

IgG
Research areas

Western blot - Anti-Arg2 antibody [EPR22626-38] (ab264066)

All lanes : Anti-Arg2 antibody [EPR22626-38] (ab264066) at 1/1000 dilution

Lane 1 : Rat kidney tissue lysate at 20 µg
Lane 2 : Rat small intestine tissue lysate at 20 µg
Lane 3 : Mouse small intestine tissue lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 39 kDa
Observed band size: 39 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Immunohistochemistry (Frozen sections) - Anti-Arg2 antibody [EPR22626-38] (ab264066)

Immunohistochemical analysis of frozen section of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse small intestine tissue labeling Arg2 with ab264066 at 1/500 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on mouse small intestine (PMID: 26895666) is observed. The nuclear counter stain is DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody [EPR22626-38] (ab264066)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Arg2/AGS with ab264066 at 1/500 dilution (1.134 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on tubules in human cerebrum. The section was incubated with ab264066 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunoprecipitation - Anti-Arg2 antibody [EPR22626-38] (ab264066)

Arg2/AGS was immunoprecipitated from 0.35 mg Mouse kidney lysate with ab264066 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab264066 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Mouse kidney lysate 10ug

Lane 2: ab264066 IP in Mouse kidney lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab264066 in Mouse kidney lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 min
Western blot - Anti-Arg2 antibody [EPR22626-38] (ab264066)

All lanes : Anti-Arg2 antibody [EPR22626-38] (ab264066) at 1/1000 dilution

Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Human kidney tissue lysate
Lane 4 : Human liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 39 kDa
Observed band size: 39 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.

Negative control: liver (PMID: 21975054).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody [EPR22626-38] (ab264066)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Arg2/AGS with ab264066 at 1/500 dilution (1.134 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in the rat colon. The section was incubated with ab264066 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody [EPR22626-38] (ab264066)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Arg2/AGS with ab264066 at 1/500 dilution (1.134 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on tubules in the rat kidney. The section was incubated with ab264066 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody [EPR22626-38] (ab264066)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Arg2/AGS with ab264066 at 1/500 dilution (1.134 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on tubules in mouse kidney. The section was incubated with ab264066 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody [EPR22626-38] (ab264066)

Immunohistochemical analysis of paraffin-embedded Mouse small intestine tissue labeling Arg2/AGS with ab264066 at 1/500 dilution (1.134 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in the mouse small intestine. The section was incubated with ab264066 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Anti-Arg2 antibody [EPR22626-38] (ab264066)

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