Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-HIBADH antibody [EPR12519(B)] (ab175203)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12519(B)] to HIBADH
Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-HIBADH antibody [EPR12519(B)]
See all HIBADH primary antibodies
Description

Rabbit monoclonal [EPR12519(B)] to HIBADH
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human HIBADH aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P31937
Positive control
- WB: HeLa and HepG2 cell lysates; Human fetal liver, fetal heart and fetal kidney tissue lysates. IHC-P: Human kidney and liver tissue. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12519(B)
Isotype

IgG
Research areas

Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203)

All lanes : Anti-HIBADH antibody [EPR12519(B)] (ab175203) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : HIBADH knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Lanes 1-3: Merged signal (red and green). Green - ab175203 observed at 35 kDa. Red - loading control ab7291 observed at 50 kDa.

ab175203 Anti-HIBADH antibody [EPR12519(B)] was shown to specifically react with HIBADH in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265888 (knockout cell lysate ab257986) was used. Wild-type and HIBADH knockout samples were subjected to SDS-PAGE. ab175203 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-HIBADH antibody [EPR12519(B)] (ab175203)

Immunofluorescent staining of HepG2 cells labeling HIBADH with ab175203 at 1/100 dilution (red). DAPI nuclear staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling HIBADH with ab175203 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-HIBADH antibody [EPR12519(B)] (ab175203)

Flow cytometric analysis of permeabilized HepG2 cells labeling HIBADH with ab175203 at 1/10 dilution (red) compared to a Rabbit IgG (green).
Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203)

All lanes : Anti-HIBADH antibody [EPR12519(B)] (ab175203) at 1/1000 dilution

Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Human fetal kidney tissue lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)

Immunohistochemical analysis of paraffin embedded Human liver tissue labeling HIBADH with ab175203 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-HIBADH antibody [EPR12519(B)] (ab175203)

Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate labeling HIBADH with ab175203 at 1/10 dilution.
Anti-HIBADH antibody [EPR12519(B)] (ab175203)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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