Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-HIBADH antibody [EPR12525(B)] (ab172955)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12525(B)] to HIBADH
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
Knockout validated
Reacts with: Human

Product name

Anti-HIBADH antibody [EPR12525(B)]
See all HIBADH primary antibodies
Description

Rabbit monoclonal [EPR12525(B)] to HIBADH
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HIBADH aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P31937
Positive control
- WB: Fetal kidney, Fetal liver, 293T, HeLa, MCF-7 and HepG2 lysates. IHC-P: Human breast carcinoma and liver tissues ICC/IF: HepG2 cells. Flow Cyt: 293T cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12525(B)
Isotype

IgG
Research areas

Western blot - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

All lanes : Anti-HIBADH antibody [EPR12525(B)] (ab172955) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : HIBADH knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Lanes 1-3: Merged signal (red and green). Green - ab172955 observed at 35 kDa. Red - loading control ab7291 observed at 50 kDa.

ab172955 Anti-HIBADH antibody [EPR12525(B)] was shown to specifically react with HIBADH in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265888 (knockout cell lysate ab257986) was used. Wild-type and HIBADH knockout samples were subjected to SDS-PAGE. ab172955 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

Immunofluorescent analysis of HepG2 cells labeling HIBADH with ab172955 at 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HIBADH with ab172955 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

Flow cytometric analysis of permeabilized 293T cells labeling HIBADH with ab172955 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Western blot - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

All lanes : Anti-HIBADH antibody [EPR12525(B)] (ab172955) at 1/10000 dilution

Lane 1 : Fetal kidney lysate
Lane 2 : Fetal liver lysate
Lane 3 : 293T lysate
Lane 4 : MCF-7 lysate
Lane 5 : HepG2 lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 35 kDa
Immunoprecipitation - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using ab172955 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling HIBADH with ab172955 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-HIBADH antibody [EPR12525(B)] (ab172955)

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