Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HIBADH antibody [EPR12525(B)] (ab172955)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12525(B)] to HIBADH
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-HIBADH antibody [EPR12525(B)]
    See all HIBADH primary antibodies

  • Description

    Rabbit monoclonal [EPR12525(B)] to HIBADH

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HIBADH aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P31937

  • Positive control

    • WB: Fetal kidney, Fetal liver, 293T, HeLa, MCF-7 and HepG2 lysates. IHC-P: Human breast carcinoma and liver tissues ICC/IF: HepG2 cells. Flow Cyt: 293T cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Western blot - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    All lanes : Anti-HIBADH antibody [EPR12525(B)] (ab172955) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : HIBADH knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab172955 observed at 35 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab172955 Anti-HIBADH antibody [EPR12525(B)] was shown to specifically react with HIBADH in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265888 (knockout cell lysate ab257986) was used. Wild-type and HIBADH knockout samples were subjected to SDS-PAGE. ab172955 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Immunocytochemistry/ Immunofluorescence - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

    Immunofluorescent analysis of HepG2 cells labeling HIBADH with ab172955 at 1/250 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HIBADH with ab172955 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Flow Cytometry - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

    Flow cytometric analysis of permeabilized 293T cells labeling HIBADH with ab172955 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

  • Western blot - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Western blot - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    All lanes : Anti-HIBADH antibody [EPR12525(B)] (ab172955) at 1/10000 dilution

    Lane 1 : Fetal kidney lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : 293T lysate
    Lane 4 : MCF-7 lysate
    Lane 5 : HepG2 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 35 kDa

  • Immunoprecipitation - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Immunoprecipitation - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

    Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using ab172955 at a 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12525(B)] (ab172955)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling HIBADH with ab172955 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-HIBADH antibody [EPR12525(B)] (ab172955)
    Anti-HIBADH antibody [EPR12525(B)] (ab172955)

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