Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1891(2)] to Bad (phospho S112)
  • Suitable for: Dot blot, WB, IP
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Bad (phospho S112) antibody [EPR1891(2)]
    See all Bad primary antibodies

  • Description

    Rabbit monoclonal [EPR1891(2)] to Bad (phospho S112)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Bad. The exact sequence is proprietary.
    (Peptide available as ab171725)

  • Positive control

    • HeLa cell lysates treated with Calyculin A.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)
    Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)
    All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/5000 dilution (purified)

    Lane 1 : Untreated C6 cells
    Lane 2 : C6 cells treated with Calcyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 18 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)
    Immunoprecipitation - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)

    ab129192 (purified) at 1/30 immunoprecipitating Bad in HeLa cell lysate treated with Calcyculin A (Lane 1). For western blotting a HRP-conjugated anti-rabbit IgG was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Dot Blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)
    Dot Blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)

    Dot blot analysis of Bad (phospho S112) using ab129192 primary antibody at a dilution of 1/1000. ab97051 was used as a secondary antibody at a dilution of 1/100,000.

    Lane 1: Bad (pS112) phospho peptide
    Lane 2: Bad non-phospho peptide

    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)
    Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)
    All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/20000 dilution (purified)

    Lane 1 : Untreated HeLa cells
    Lane 2 : HeLa cells treated with Calcyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 18 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)
    Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)
    All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/1000 dilution

    Lane 1 : HeLa cell lysates, untreated
    Lane 2 : HeLa cell lysates treated with Calyculin A.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 18 kDa

  • OI-RD Scanning - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)
    OI-RD Scanning - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)
    Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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