Anti-Bad antibody (ab90435)
Key features and details
- Rabbit polyclonal to Bad
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Bad antibody
See all Bad primary antibodies -
Description
Rabbit polyclonal to Bad -
Host species
Rabbit -
Specificity
This antibody specifically binds the Bad protein, but does not recognize Bad/Bcl2 heterodimers in Immunoprecipitation assays. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide derived from the sequence of Mouse Bad, conjugated to KLH.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Bad antibody (ab90435)Anti-Bad antibody (ab90435) at 1 µg/ml + Mouse Raw 264 cell lysate
Developed using the ECL technique.
Predicted band size: 18 kDa
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Western blot - Anti-Bad antibody (ab90435) This image is courtesy of an anonymous AbreviewAll lanes : Anti-Bad antibody (ab90435) at 1/1000 dilution
Lanes 1 & 5 : Cow normal mammary epithelial cells (nMEC) treated with serum free media
Lanes 2 & 6 : Cow normal mammary epithelial cells (nMEC) treated with 1.0µM anisomycin
Lanes 3 & 7 : Cow normal mammary epithelial cells (nMEC) treated with 100ng/ml IGF-1
Lanes 4 & 8 : Cow normal mammary epithelial cells (nMEC) treated with anisomycin and IGF-1
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : ECL Anti-Rabbit IgG, Horseradish Peroxidase Linked at 2500 cells
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 30 secondsTreatement for 30 minutes (lanes 1-4) or for 1 hour (lanes 5-8). 12.5% SDS-PAGE. Proteins collected in RIPA Buffer and transferred to PVDF (0.45 um).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad antibody (ab90435)ab90435, at a 1/50 dilution, staining BAD in the neurons of paraffin embedded Alzheimer diseased brain tissue (right) by Immunohistochemistry. Control brain tissue (left).
