Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Arg2 antibody [EPR22626-51] - BSA and Azide free (ab255967)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22626-51] to Arg2 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Arg2 antibody [EPR22626-51] - BSA and Azide free
    See all Arg2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22626-51] to Arg2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat small intestine and kidney lysate. IHC-P: Mouse small intestine tissue. Rat kidney tissue. IP: Rat kidney lysate.

  • General notes

    Ab255967 is the carrier-free version of ab228963. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255967 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody [EPR22626-51] - BSA and Azide free (ab255967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody [EPR22626-51] - BSA and Azide free (ab255967)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling Arg2 with ab228963 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the mouse small intestine (PMID: 19000307) is observed. The section was incubated with ab228963 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228963).

  • Immunoprecipitation - Anti-Arg2 antibody [EPR22626-51] - BSA and Azide free (ab255967)
    Immunoprecipitation - Anti-Arg2 antibody [EPR22626-51] - BSA and Azide free (ab255967)

    Arg2 was immunoprecipitated from 0.35 mg rat kidney lysate with ab228963 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab228963 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.

    Lane 1: Rat kidney  lysate 10 µg (Input).
    Lane 2: ab228963 IP in rat kidney lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab228963 in rat kidney lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228963).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody [EPR22626-51] - BSA and Azide free (ab255967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody [EPR22626-51] - BSA and Azide free (ab255967)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Arg2 with ab228963 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the tubules in the rat kidney (PMID: 8954792) is observed. The section was incubated with ab228963 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228963).

  • Anti-Arg2 antibody [EPR22626-51] - BSA and Azide free (ab255967)
    Anti-Arg2 antibody [EPR22626-51] - BSA and Azide free (ab255967)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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