Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Serum Amyloid A antibody [EP11592-92] - BSA and Azide free (ab235995)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP11592-92] to Serum Amyloid A - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Serum Amyloid A antibody [EP11592-92] - BSA and Azide free
    See all Serum Amyloid A primary antibodies

  • Description

    Rabbit monoclonal [EP11592-92] to Serum Amyloid A - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: Human liver cancer tissue; WB: Human plasma lysate.

  • General notes

    Ab235995 is the carrier-free version of ab190802. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab235995 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Serum Amyloid A antibody [EP11592-92] - BSA and Azide free (ab235995)
    Western blot - Anti-Serum Amyloid A antibody [EP11592-92] - BSA and Azide free (ab235995)
    Anti-Serum Amyloid A antibody [EP11592-92] (ab190802) at 1/1000 dilution (Purified) + Human plasma lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 14 kDa
    Observed band size: 12 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab190802, the same antibody clone in a different buffer formulation.

    Blocking buffer: 5% NFDM/TBST

  • Immunohistochemistry paraffin embedded sections - Anti-Serum Amyloid A antibody [EP11592-92] - BSA and Azide free (ab235995)
    Immunohistochemistry paraffin embedded sections - Anti-Serum Amyloid A antibody [EP11592-92] - BSA and Azide free (ab235995)
    This data was developed using ab190802 the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue sections labeling Serum Amyloid A with Purified ab190802 at 1:400 dilution (0.96 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Serum Amyloid A antibody [EP11592-92] - BSA and Azide free (ab235995)

© 2021 Astana Biomed Group LLP. All rights reserved.