Human Thrombin ELISA Kit (Factor II) (ab108909)
Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.313 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Thrombin ELISA Kit (Factor II) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.7% Inter-assay Sample n Mean SD CV% Overall 7.2% -
Sample type
Cell culture supernatant -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.3 ng/ml -
Range
0.313 ng/ml - 20 ng/ml -
Recovery
98 %
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Assay time
4h 0m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Thrombin (Factor II) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombin concentrations in cell culture supernatants. This assay recognizes both natural and recombinant Human thrombin.
A Thrombin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Thrombin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Thrombin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 20X Wash Buffer Concentrate 2 x 30ml 80X Biotinylated Human Thrombin Antibody 1 x 75µl Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml Thrombin Microplate (12 x 8 well strips) 1 unit Thrombin Standard 1 vial -
Research areas
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Database links
- SwissProt: P00734 Human
Images
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Typical Standard Curve
Representative Standard Curve Using ab108909.