Mouse SAA ELISA Kit (ab157723)
Key features and details
- Sensitivity: 10.7 ng/ml
- Range: 31.25 ng/ml - 2000 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse SAA ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10.7 ng/ml -
Range
31.25 ng/ml - 2000 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse SAA ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Serum Amyloid A in mouse samples.
In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Serum Amyloid A. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Serum Amyloid A in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-conjugated anti-mouse SAA antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Mouse SAA Calibrator (lyophilized) 1 vial Mouse SAA ELISA Microplate 1 unit Stop Solution 1 x 12ml -
Research areas
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Relevance
The serum amyloid A (SAA) family comprises a number of differentially expressed lipoproteins, acute phase SAA1 and SAA2, the former being a major component in plasma, and constitutive SAA's (C-SAAs). Although the liver is the primary site of synthesis of both SAA types, extrahepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed it's importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations. SAA rises earlier and more sharply than CRP. SAA enhances the binding of HDL's to macrophages and thus helps the delivery of lipid to sites of injury for use in tissue repair. It is thus thought to be an integral part of the disease process. In addition, recent experiments suggest that SAA may play a "houekeeping" role in normal human tissues. Elevated levels of SAA over time predispose secondary amyloidosis, extracellular accumulation of amyloid fibrils, derived from a circulating precursor, in various tissues and organs. The most common form of amyloidosis occurs secondary to chronic inflammatory disease, particularly rheumatoid artheritis. -
Cellular localization
Secreted -
Alternative names
- Amyloid fibril protein AA
- Amyloid protein A
- MGC111216
see all -
Database links
- Entrez Gene: 20208 Mouse
- SwissProt: P05366 Mouse
- Unigene: 148800 Mouse
Images
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ab157723 SAA Mouse ELISA Kit
SAA measured in various biological samples showing quantity (ng) per mL of sample tested. For mouse samples (Ms) dilutions of 1:1000 were used.
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Typical Standard CurveRepresentative standard curve using ab157723 SAA Mouse ELISA Kit.
