Canine SAA ELISA Kit (ab205072)
Key features and details
- Range: 6.25 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine SAA ELISA Kit
See all Serum Amyloid A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Range
6.25 ng/ml - 200 ng/ml -
Recovery
> 85 %
Sample specific recovery Sample type Average % Range Serum > 85 6.25ng/ml - 200ng/ml -
Assay time
1h 40m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
SAA in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of SAA protein in dog cell culture supernatant, plasma, serum and urine.
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Notes
In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP Conjugated Enzyme Antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentration 1 x 50ml Anti-Dog SAA ELISA Microplate 1 unit Chromogen Substrate Solution 1 x 12ml Dog SAA Calibrator 1 vial Stop Solution 1 x 12ml -
Research areas
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Relevance
Function: Major acute phase reactant. Apolipoprotein of the HDL complex. Tissue specificity: Expressed by the liver; secreted in plasma. Disease: Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Note=Elevated serum SAA protein levels may be associated with lung cancer. Similarity: Belongs to the SAA family. PTM: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end. -
Alternative names
- Amyloid fibrils
- SAA 2
- Serum amyloid A1 isoform 2
see all -
Database links
Images
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Standard Curve
Representative standard curve using ab205072 Dog SAA ELISA Kit.