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Cancer Cancer Metabolism Response to hypoxia

Anti-HIGD2A antibody (ab150893)

Anti-HIGD2A antibody (ab150893)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to HIGD2A
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-HIGD2A antibody
    See all HIGD2A primary antibodies
  • Description

    Rabbit polyclonal to HIGD2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to amino acids 12-46 of Human HIGD2A (Q9BW72).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HEK-293 whole cell lysates; IHC-P: Human kidney tissue; ICC: MCF7 whole cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cancer
    • Cancer Metabolism
    • Response to hypoxia

Images

  • Western blot - Anti-HIGD2A antibody (ab150893)
    Western blot - Anti-HIGD2A antibody (ab150893)
    Anti-HIGD2A antibody (ab150893) at 0.4 µg/ml + HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

    Predicted band size: 11 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIGD2A antibody (ab150893)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIGD2A antibody (ab150893)

    Immunohistochemical analysis of human kidney tissue labeling HIGD2A in the granular cytoplasm of cells in tubules with ab150893 at a 1/20 dilution.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-HIGD2A antibody (ab150893)
    Immunocytochemistry/ Immunofluorescence - Anti-HIGD2A antibody (ab150893)

    Immunocytochemical analysis of MCF7 (Human breast adenocarcinoma cell line) whole cells labeling HIGD2A in the cytosol and mitochondria with ab150893 at 2 µg/ml.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-HIGD2A antibody (ab150893)

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    Applications: ICC/IF, WB

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