Mouse IgG1 ELISA Kit (ab133045)
Key features and details
- Sensitivity: 0.064 ng/ml
- Range: 7.81 ng/ml - 250 ng/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IgG1 ELISA Kit
See all IgG1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Low 20 46.2pg/ml 1.3% Medium 20 95.1pg/ml 3.2% High 20 147pg/ml 5% Inter-assay Sample n Mean SD CV% Low 7 47.3pg/ml 4.4% Medium 7 101pg/ml 4.8% High 7 152pg/ml 4.5% -
Sample type
Cell culture supernatant, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
0.064 ng/ml -
Range
7.81 ng/ml - 250 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 102.8 % - % Tissue Culture Media 105.3 % - % -
Assay time
0h 90m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s Mouse IgG1in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Mouse IgG1 in Culture supernatants and Serum.
IgG1 specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated IgG1 detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the IgG1 amount of sample captured in plate.
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Notes
IgG is divided into four subclasses; IgG1, IgG2, IgG3, and IgG4. IgG1 is the most abundant immunoglobulin found in the blood. It is a glycoprotein which consists of two identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig) domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the “hinge region” which is unique to each IgG. This region allows for molecular flexibility and sets IgG1 apart from its IgG counterparts. IgG1 properties and functions include neutralization, opsonization, activation of the complement system, diffusion into extravascular sites and crossing the placenta.
Cross Reactivity
Compound Cross Reactivity Rat IgG1 0.9% Mouse IgG2b 0.21 Human IgG1 Mouse IgG2a Mouse IgG3 Mouse IgM Recommended dilution of serum is 1:20,000
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 100ml Assay Buffer 13 Concentrate 1 x 50ml Goat anti-mouse IgG Microplate (12 x 8 wells) 1 unit Mouse IgG1 Horseradish Peroxidase Conjugate 1 x 6ml Mouse IgG1 Standard 1 x 0.25ml Plate Sealer 2 units Stop Solution 2 1 x 11ml TMB Substrate 1 x 12ml -
Research areas
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Relevance
There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). -
Alternative names
- Ig gamma 1 chain C region
- IGHG1
Images
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Typical Standard Curve
Representative Standard Curve using ab133045.