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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Other ECM Proteins

Recombinant human Trefoil Factor 3 protein (ab82102)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

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Description

  • Product name

    Recombinant human Trefoil Factor 3 protein
    See all Trefoil Factor 3 proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    Purity is greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Endotoxin level

  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF
  • Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab82102 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        Functional Studies

        SDS-PAGE

      • Form

        Lyophilized
      • Additional notes

        Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8oC for up to 1 week. For
        extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

      General Info

      • Alternative names

        • hITF
        • hP1.B
        • Intestinal trefoil factor
        • ITF
        • mITF
        • OTTMUSP00000021729
        • P1B
        • Polypeptide P1.B
        • TFF3
        • TFF3_HUMAN
        • TFI
        • TREFOIL
        • Trefoil factor (intestinal)
        • Trefoil factor 3
        see all
      • Function

        Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
      • Tissue specificity

        Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.
      • Sequence similarities

        Contains 1 P-type (trefoil) domain.
      • Cellular localization

        Secreted > extracellular space > extracellular matrix > apical lamina.
      • Target information above from: UniProt accession Q07654 The UniProt Consortium
        The Universal Protein Resource (UniProt) in 2010
        Nucleic Acids Res. 38:D142-D148 (2010) .

        Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab82102? Please let us know so that we can cite the reference in this datasheet.

    ab82102 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Alternative names

      • hITF
      • hP1.B
      • Intestinal trefoil factor
      • ITF
      • mITF
      • OTTMUSP00000021729
      • P1B
      • Polypeptide P1.B
      • TFF3
      • TFF3_HUMAN
      • TFI
      • TREFOIL
      • Trefoil factor (intestinal)
      • Trefoil factor 3
      see all
    • Function

      Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
    • Tissue specificity

      Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.
    • Sequence similarities

      Contains 1 P-type (trefoil) domain.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix > apical lamina.
    • Target information above from: UniProt accession Q07654 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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