Anti-Fibulin 2 antibody (ab234993)
Key features and details
- Rabbit polyclonal to Fibulin 2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Fibulin 2 antibody
See all Fibulin 2 primary antibodies -
Description
Rabbit polyclonal to Fibulin 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human Fibulin 2 aa 300-450.
Database link: P98095 -
Positive control
- WB: HeLa, U-251 MG and A549 whole cell lysates. IHC-P: Human placenta and liver cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 2 antibody (ab234993)
Paraffin-embedded human placenta tissue stained for Fibulin 2 using ab234993 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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Western blot - Anti-Fibulin 2 antibody (ab234993)All lanes : Anti-Fibulin 2 antibody (ab234993) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 127 kDa
Observed band size: 127 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 2 antibody (ab234993)Paraffin-embedded human liver cancer tissue stained for Fibulin 2 using ab234993 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
