Mouse TFF3 ELISA Kit (ab253228)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.9 pg/ml
- Range: 7.81 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse TFF3 ELISA Kit
See all Trefoil Factor 3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 8 4.5% Inter-assay Sample n Mean SD CV% Plasma 3 2.8% -
Sample type
Cell culture supernatant, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.9 pg/ml -
Range
7.81 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 84 82% - 85% Hep Plasma 93 85% - 99% EDTA Plasma 87 82% - 90% Cit plasma 95 87% - 101% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse TFF3 ELISA Kit (ab253228) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TFF3 protein in cit plasma, edta plasma, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TFF3 with 2.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Trefoil factor 3 is a protein that is encoded by the TFF3 gene. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to anti-apoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. TFF3 is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies. Mouse TFF3 shares 85.2%, 94.8%, 82.5%, and 72.9% sequence homology with human, rat, bovine, and primate respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse TFF3 Capture Antibody 1 x 600µl 10X Mouse TFF3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse TFF3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen). -
Tissue specificity
Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. -
Sequence similarities
Contains 1 P-type (trefoil) domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix > apical lamina. - Information by UniProt
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Alternative names
- hITF
- hP1.B
- Intestinal trefoil factor
see all -
Database links
- Entrez Gene: 21786 Mouse
- SwissProt: Q62395 Mouse
- Unigene: 4641 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse TFF3 standard curve in Sample Diluent NS.The TFF3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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. Interpolated concentrations of native TFF3 in mouse plasma and cell samples. The concentrations of TFF3 were measured in duplicates,The concentrations of TFF3 were measured in duplicates, interpolated from the TFF3 standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 20%, plasma (EDTA) 20%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFF3 concentration was determined to be 1599.11 pg/mL plasma (citrate) in 2601.71 pg/mL in plasma (citrate), and 2822.63 pg/mL in plasma (heparin).
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Sandwich ELISA - Mouse TFF3 ELISA Kit (ab253228)To learn more about the advantages of recombinant antibodies see here.
