Human Ceruloplasmin Antibody Pair - BSA and Azide free (ab244149)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
-
Product name
Human Ceruloplasmin Antibody Pair - BSA and Azide free
See all Ceruloplasmin kits -
Assay type
ELISA set -
Range
230 pg/ml - 15000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human Ceruloplasmin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab236718), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
-
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Ceruloplasmin Capture Antibody (unconjugated) 1 x 100µg Human Ceruloplasmin Detector Antibody (unconjugated) 1 x 100µg -
Research areas
-
Function
Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. -
Sequence similarities
Belongs to the multicopper oxidase family.
Contains 3 F5/8 type A domains.
Contains 6 plastocyanin-like domains. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- CERU_HUMAN
- Ceruloplasmin
- CP
see all -
Database links
- Entrez Gene: 1356 Human
- Omim: 117700 Human
- SwissProt: P00450 Human
- Unigene: 558314 Human
Images
-
Sandwich ELISA - Human Ceruloplasmin Antibody Pair - BSA and Azide free (ab244149)To learn more about the advantages of recombinant antibodies see here.