Anti-Huntingtin (phospho S421) antibody (ab2174)
Key features and details
- Rabbit polyclonal to Huntingtin (phospho S421)
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Huntingtin (phospho S421) antibody
See all Huntingtin primary antibodies -
Description
Rabbit polyclonal to Huntingtin (phospho S421)This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Rabbit -
Specificity
Detects a 190kDa band in PC-3 cells, which may correspond to Huntingtin. Stains neuronal cytoplasm in immunohistochemistry (the expected localisation). The antibody has not been conclusively tested for specificity for phospho S421 Huntingtin. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide: RSRSGpSIVE, corresponding to amino acids 416-424 of Human Huntingtin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Huntingtin (phospho S421) antibody (ab2174)Anti-Huntingtin (phospho S421) antibody (ab2174) at 1/1000 dilution + human PC-3 cell lysate
Predicted band size: 348 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 130 kDa. We are unsure as to the identity of these extra bands.
The 190kDa band may correspond to Huntingtin. However, it is not clear whether the antibody is specific for the phospho S421 form. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Huntingtin (phospho S421) antibody (ab2174)ab2174 was used at a 1:100 dilution to detect phosphorylated Huntingtin by immunohistochemistry in human brain cerebellum. Positive cytoplasmic staining is observed in neurons. Tissue was formalin-fixed and paraffin embedded. Detection was with AEC (pink), nuclear counterstaining with Haemotoxylin (blue).
