Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834)
![Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834)](https://www.abcam.com/ps/products/190/ab190834/Images/ab190834-286575-anti-mannan-binding-lectin-antibody-epr18381-156-western-blot.jpg "Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18381-156] to Mannan Binding Lectin/MBL
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156]
See all Mannan Binding Lectin/MBL primary antibodies -
Description
Rabbit monoclonal [EPR18381-156] to Mannan Binding Lectin/MBL -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseHumanWB Mouse
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse liver and kidney lysates; Mouse serum; Mouse Mannan Binding Lectin/MBL (aa19-244) recombinant protein. IHC-P: Mouse and human liver tissues.
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General notes
This product was previously labelled as Mannan Binding Lectin
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18381-156 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834)Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834) at 1/1000 dilution + Mouse Mannan Binding Lectin/MBL (aa19-244) recombinant protein at 0.005 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 5 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834)](https://www.abcam.com/ps/products/190/ab190834/Images/ab190834-286576-anti-mannan-binding-lectin-antibody-epr18381-156-western-blot.jpg)
Western blot - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834)All lanes : Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse serum
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDaExposure time : Lane 1: 3 seconds; Lane 2: 3 minutes; Lane 3 1 second.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834)](https://www.abcam.com/ps/products/190/ab190834/Images/ab190834-286577-anti-mannan-binding-lectin-antibody-epr18381-156-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Mannan Binding Lectin/MBL with ab190834 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse liver is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)(ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834)](https://www.abcam.com/ps/products/190/ab190834/Images/ab190834-286578-anti-mannan-binding-lectin-antibody-epr18381-156-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Mannan Binding Lectin/MBL with ab190834 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human liver is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)(ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834)](https://www.abcam.com/ps/products/190/ab190834/Images/ab190834-5-benefits-of-recombinant-antibodies.png)
Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834)
