Human MBL ELISA Kit (Mannose-Binding Lectin) (ab193709)
Key features and details
- Sensitivity: 0.03 ng/ml
- Range: 0.03 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MBL ELISA Kit (Mannose-Binding Lectin)
See all Mannan Binding Lectin/MBL kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.03 ng/ml -
Range
0.03 ng/ml - 25 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 122.8 103% - 147% Plasma 121.1 98% - 145% Cell culture media 103.1 91% - 116% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s MBL (Mannose-Binding Lectin) Human ELISA Kit (ab193709) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MBL in sera, plasma and cell culture supernatants.
This assay employs an antibody specific for Human MBL coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures MBL present in the samples. The wells are washed and biotinylated anti-Human MBL antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of MBL bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated Human MBL detection antibody (lyophilized) 2 vials Human MBL Standards (lyophilized) 2 vials Pre-coated Human MBL Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. -
Tissue specificity
Plasma protein produced mainly in the liver. -
Involvement in disease
Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- COLEC 1
- COLEC1
- Collectin-1
see all -
Database links
- Entrez Gene: 4153 Human
- Omim: 154545 Human
- SwissProt: P11226 Human
- Unigene: 499674 Human
Images
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Typical standard curve in Assay Diluent A
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Typical standard curve in Assay Diluent BData provided for demonstration purposes only. A new standard curve must be generated for each assay performed.