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Immunology Innate Immunity Complement Alternative Pathway

Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)

Price and availability

271 382 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17004-285] to Factor D/CFD
  • Suitable for: WB
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-Factor D/CFD antibody [EPR17004-285]
    See all Factor D/CFD primary antibodies
  • Description

    Rabbit monoclonal [EPR17004-285] to Factor D/CFD
  • Host species

    Rabbit
  • Specificity

    This antibody detects a distinct epitope compared to ab213177.
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged Mouse Factor D/CFD (aa26-259) recombinant protein. Mouse plasma, serum and lung lysate.
  • General notes

     This product was previously labelled as Factor D

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17004-285
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Complement
    • Alternative Pathway

Images

  • Western blot - Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)
    Western blot - Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)
    Anti-Factor D/CFD antibody [EPR17004-285] (ab181307) at 1/100000 dilution + His-tagged Mouse Factor D/CFD (aa26-259) recombinant protein 10 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 27 kDa
    Observed band size: 29 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.

     

  • Western blot - Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)
    Western blot - Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)
    All lanes : Anti-Factor D/CFD antibody [EPR17004-285] (ab181307) at 1/1000 dilution

    Lane 1 : Mouse plasma lysate
    Lane 2 : Mouse serum lysate
    Lane 3 : Mouse lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 27 kDa
    Observed band size: 45-50 kDa why is the actual band size different from the predicted?



    Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.

    Exposure times:

    Lane 1/2: 1 second

    Lane 3: 15 seconds

    The higher molecular mass corresponds to glycosylated forms of the protein. The expression profile observed is consistent with what has been described in the literature (PMID: 20038603; PMID: 12949072).

  • Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)
    Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)

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    Applications: IHC-P

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    Applications:

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