Anti-EXOSC10/RRP6 antibody (ab264343)
Key features and details
- Rabbit polyclonal to EXOSC10/RRP6
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EXOSC10/RRP6 antibody
See all EXOSC10/RRP6 primary antibodies -
Description
Rabbit polyclonal to EXOSC10/RRP6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human EXOSC10/RRP6 aa 750-800. The exact sequence is proprietary. NP_001001998.1
Database link: Q01780 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whle cell lysates. IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-EXOSC10/RRP6 antibody (ab264343) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 101 kDa
Exposure time: 30 seconds
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EXOSC10/RRP6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264343 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264343 at 1 μg/ml.
Lane 1: ab264343 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC10/RRP6 antibody (ab264343)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for EXOSC10/RRP6 using ab264343 at 1/1000 dilution in immunohistochemical analysis. DAB staining.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.