Human Factor D ELISA Kit (ab99969)
Key features and details
- Sensitivity: 4 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-human Adipsin 2 vials Factor D Microplate (12 strips x 8 wells) 1 unit Recombinant human Fatcor D Standards (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. -
Involvement in disease
Defects in CFD are the cause of complement factor D deficiency (CFD deficiency) [MIM:134350]. CFD deficiency predisposes to invasive meningococcal disease. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Adipsin
- Adipsin/complement factor D
- Adn
see all -
Database links
- Entrez Gene: 1675 Human
- Omim: 134350 Human
- SwissProt: P00746 Human
- Unigene: 155597 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab99969.
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Typical Standard CurveRepresentative Standard Curve using ab99969.
