Human Complement Factor D ELISA Kit (ab243686)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.6 pg/ml
- Range: 15.63 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Complement Factor D ELISA Kit
See all Factor D/CFD kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 2.4% Inter-assay Sample n Mean SD CV% serum 3 4.3% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.6 pg/ml -
Range
15.63 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 96 87% - 102% Urine 88 83% - 90% Serum 98 92% - 105% Hep Plasma 95 82% - 102% EDTA Plasma 93 84% - 102% Cit plasma 94 90% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Complement Factor D ELISA Kit (ab243686) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Complement Factor D protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Complement Factor D with 2.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Complement Factor D Capture Antibody 1 x 600µl 10X Human Complement Factor D Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Complement Factor D Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. -
Involvement in disease
Defects in CFD are the cause of complement factor D deficiency (CFD deficiency) [MIM:134350]. CFD deficiency predisposes to invasive meningococcal disease. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Adipsin
- Adipsin/complement factor D
- Adn
see all -
Database links
- Entrez Gene: 1675 Human
- Omim: 134350 Human
- SwissProt: P00746 Human
- Unigene: 155597 Human
Images
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Other - Human Complement Factor D ELISA Kit (ab243686)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Complement Factor D Standard Curve Comparison.Standard Curve comparison between human Complement Factor D SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Sandwich ELISA - Human Complement Factor D ELISA Kit (ab243686)Example of human Complement Factor D standard curve in Sample Diluent NS.
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Interpolated concentrations of native Complement Factor D in human serum and plasma samples.The concentrations of Complement Factor D were measured in duplicates, interpolated from the Complement Factor D standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1,000, plasma (citrate) 1:1,000, plasma (EDTA) 1:1,000and plasma (heparin) 1:1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor D concentration was determined to be 944.1 ng/mL in serum, 721.8 ng/mL in plasma (citrate), 860.5 ng/mL in plasma (EDTA), and 321.7 ng/mL in plasma (heparin).
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Interpolated concentrations of native Complement Factor D in human urine and cell culture supernatant samples.The concentrations of Complement Factor D were measured in duplicates, interpolated from the Complement Factor D standard curves and corrected for sample dilution. Undiluted samples are as follows: male urine 100%, female urine 50%, and U937 supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor D concentration was determined to be 958.8 pg/mL in male urine, 494.7 pg/mL in female urine and 4,734.2 pg/mL in U937 supernatant.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor D concentration was determined to be 975.9 ng/mL with a range of 556.8 – 1,603.5 ng/mL.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor D concentration was determined to be 1,142.5 ng/mL with a range of 867.1 – 1,958.8 ng/mL.
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Sandwich ELISA - Human Complement Factor D ELISA Kit (ab243686)To learn more about the advantages of recombinant antibodies see here.
