Anti-SMARCC1/BAF155 antibody (ab72503)
Key features and details
- Rabbit polyclonal to SMARCC1/BAF155
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SMARCC1/BAF155 antibody
See all SMARCC1/BAF155 primary antibodies -
Description
Rabbit polyclonal to SMARCC1/BAF155 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
-
Immunogen
Synthetic peptide corresponding to Human SMARCC1/BAF155 (C terminal).
Database link: NP_003065.2 -
Positive control
- HeLa whole cell lysate (ab150035).
-
General notes
Previously labelled as SMARCC1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab72503 was affinity purified using an epitope specific to SMARCC1/BAF155 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-SMARCC1/BAF155 antibody (ab72503)All lanes : Anti-SMARCC1/BAF155 antibody (ab72503) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 123 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 1 second
-
Immunoprecipitation - Anti-SMARCC1/BAF155 antibody (ab72503)Detection of SMARCC1/BAF155 by Western Blot of Immunprecipitate.
ab72503 at 1µg/ml staining SMARCC1/BAF155 in HeLa whole cell lysate immunoprecipitated using ab72503 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 1 second. -
Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody (ab72503)ICC/IF image of ab72503 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72503, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
