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Signal Transduction Metabolism Plasma Membrane ATPases

Anti-ATP1B2 antibody [EPR15461(B)] - BSA and Azide free (ab240290)

Anti-ATP1B2 antibody [EPR15461(B)] - BSA and Azide free (ab240290)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15461(B)] to ATP1B2 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-ATP1B2 antibody [EPR15461(B)] - BSA and Azide free
    See all ATP1B2 primary antibodies
  • Description

    Rabbit monoclonal [EPR15461(B)] to ATP1B2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human cerebellum lysate.
  • General notes

    ab240290 is the carrier-free version of ab185207.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15461(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases

Images

  • Immunoprecipitation - Anti-ATP1B2 antibody [EPR15461(B)] - BSA and Azide free (ab240290)
    Immunoprecipitation - Anti-ATP1B2 antibody [EPR15461(B)] - BSA and Azide free (ab240290)

    Western blot analysis of ATP1B2 in immunoprecipitation pellets from Human Y79 lysate. Lane 1 ab185207 used at 1/50 dilution. Lane 2 PBS negative control Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185207).

  • Western blot - Anti-ATP1B2 antibody [EPR15461(B)] - BSA and Azide free (ab240290)
    Western blot - Anti-ATP1B2 antibody [EPR15461(B)] - BSA and Azide free (ab240290)
    Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207) at 1/20000 dilution + Human cerebellum lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 33 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185207).

  • Anti-ATP1B2 antibody [EPR15461(B)] - BSA and Azide free (ab240290)
    Anti-ATP1B2 antibody [EPR15461(B)] - BSA and Azide free (ab240290)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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