Anti-SMARCC1/BAF155 antibody [EPR12389] - BSA and Azide free (ab249725)
![Anti-SMARCC1/BAF155 antibody [EPR12389] - BSA and Azide free (ab249725)](https://www.abcam.com/ps/products/249/ab249725/Images/ab249725-406044-anti-smarcc1-baf155-antibody-epr12389-bsa-and-azide-free-western-blot-human.jpg "Anti-SMARCC1/BAF155 antibody [EPR12389] - BSA and Azide free (ab249725)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12389] to SMARCC1/BAF155 - BSA and Azide free
- Suitable for: ICC, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SMARCC1/BAF155 antibody [EPR12389] - BSA and Azide free
See all SMARCC1/BAF155 primary antibodies -
Description
Rabbit monoclonal [EPR12389] to SMARCC1/BAF155 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249725 is the carrier-free version of ab172636 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249725 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR12389 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMARCC1/BAF155 antibody [EPR12389] - BSA and Azide free (ab249725)All lanes : Anti-SMARCC1/BAF155 antibody [EPR12389] (ab172636) at 1/5000 dilution
Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : SMARCC1 knockout HEK-293 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 123 kDa
Observed band size: 123 kDaThis data was developed using ab172636, the same antibody clone in a different buffer formulation.
Lanes 1 - 4: Merged signal (red and green). Green - ab172636 observed at 123 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab172636 was shown to specifically react with SMARCC1 in wild-type HEK-293 cells as signal was lost in SMARCC1 knockout cells. Wild-type and SMARCC1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab172636 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry - Anti-SMARCC1/BAF155 antibody [EPR12389] - BSA and Azide free (ab249725)](https://www.abcam.com/ps/products/249/ab249725/Images/ab249725-2-anti-smarcc1-baf155-antibody-epr12389-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-SMARCC1/BAF155 antibody [EPR12389] - BSA and Azide free (ab249725) -
![Western blot - Anti-SMARCC1/BAF155 antibody [EPR12389] - BSA and Azide free (ab249725)](https://www.abcam.com/ps/products/249/ab249725/Images/ab249725-398783-anti-smarcc1-baf155-antibody-epr12389-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-SMARCC1/BAF155 antibody [EPR12389] - BSA and Azide free (ab249725)All lanes : Anti-SMARCC1/BAF155 antibody [EPR12389] (ab172636) at 1/1000 dilution
Lane 1 : NTera2/D1 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 123 kDaThis data was developed using ab172636, the same antibody clone in a different buffer formulation.
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![Anti-SMARCC1/BAF155 antibody [EPR12389] - BSA and Azide free (ab249725)](https://www.abcam.com/ps/products/249/ab249725/Images/ab249725-4-benefits-of-recombinant-antibodies.png)
Anti-SMARCC1/BAF155 antibody [EPR12389] - BSA and Azide free (ab249725)
