Anti-Prealbumin antibody [EPR24246-14] (ab271132)
![Anti-Prealbumin antibody [EPR24246-14] (ab271132)](https://www.abcam.com/ps/products/271/ab271132/Images/ab271132-451599-anti-prealbumin-antibody-epr2424614-western-blot-ul.jpg "Anti-Prealbumin antibody [EPR24246-14] (ab271132)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24246-14] to Prealbumin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Prealbumin antibody [EPR24246-14]
See all Prealbumin primary antibodies -
Description
Rabbit monoclonal [EPR24246-14] to Prealbumin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatWB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human, mouse and rat plasma. Rat liver tissue lysate. IHC-P: Mouse and rat cerebrum tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24246-14 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Prealbumin antibody [EPR24246-14] (ab271132)All lanes : Anti-Prealbumin antibody [EPR24246-14] (ab271132) at 1/1000 dilution
Lane 1 : Human plasma
Lane 2 : Rat plasma
Lysates/proteins at 20 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure times: Lane 1: 15 seconds; Lane 2: 1 second
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody (ab271132)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Prealbumin with ab271132 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the choroid plexus of mouse cerebrum (PMID: 29527688). The section was incubated with ab271132 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Western blot - Anti-Prealbumin antibody [EPR24246-14] (ab271132)](https://www.abcam.com/ps/products/271/ab271132/Images/ab271132-451600-anti-prealbumin-antibody-epr2424614-western-blot-ul1.jpg)
Western blot - Anti-Prealbumin antibody [EPR24246-14] (ab271132)Anti-Prealbumin antibody [EPR24246-14] (ab271132) at 1/1000 dilution + Mouse plasma at 20 µl
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds
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Western blot - Anti-Prealbumin antibody (ab271132)All lanes : Anti-Prealbumin antibody [EPR24246-14] (ab271132) at 1/1000 dilution
Lane 1 : Rat liver tissue lysate
Lane 2 : Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: skeletal muscle (PMID: 19032346).
Exposure time: 15 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody (ab271132)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Prealbumin with ab271132 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the choroid plexus of rat cerebrum. The section was incubated with ab271132 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody (ab271132)Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Prealbumin with ab271132 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab271132 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control: no staining on rat skeletal muscle.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.