Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free (ab210608)
![Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free (ab210608)](https://www.abcam.com/ps/products/210/ab210608/Images/ab210608-385413-antivegfreceptor1antibodyy103immunohistochemistrygastriccarcinomahuman.jpg "Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free (ab210608)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y103] to VEGF Receptor 1 - BSA and Azide free
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free
See all VEGF Receptor 1 primary antibodies -
Description
Rabbit monoclonal [Y103] to VEGF Receptor 1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab182457) -
Positive control
- IP: Mouse brain lysate. IHC-P: Human gastric carcinoma tissue.
-
General notes
ab210608 is the carrier-free version of ab32152. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y103 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free (ab210608)
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue labeling VEGF with ab32152, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human gastric carcinoma. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab32152).
-
![Immunoprecipitation - Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free (ab210608)](https://www.abcam.com/ps/products/210/ab210608/Images/ab210608-385412-antivegfreceptor1antibodyy103immunoprecipitationbrainmouse.jpg)
Immunoprecipitation - Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free (ab210608)VEGF Receptor 1 was immunoprecipitated from 0.35 mg mouse brain lysate 10μg with ab32152 at 1:30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab32152 1:1000 dilution (2 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
Lane 1: Mouse brain lysate 10μg.
Lane 2: ab32152 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab32152 in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab32152).
-
![Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free (ab210608)](https://www.abcam.com/ps/products/210/ab210608/Images/ab210608-4-benefits-of-recombinant-antibodies.png)
Anti-VEGF Receptor 1 antibody [Y103] - BSA and Azide free (ab210608)
