Anti-VEGF Receptor 1 antibody (ab2350)
Key features and details
- Rabbit polyclonal to VEGF Receptor 1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VEGF Receptor 1 antibody
See all VEGF Receptor 1 primary antibodies -
Description
Rabbit polyclonal to VEGF Receptor 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Pig
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Immunogen
Synthetic peptide within Human VEGF Receptor 1 aa 1300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P17948 -
Positive control
- IHC-P: Human angiosarcoma tissue. ICC/IF: A431 cell line
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 1 antibody (ab2350)
ICC/IF image of ab2350 stained A431 (human epidermoid carcinoma cell line) cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2350, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody (ab2350)ab2350 - immunohistochemistry
Formalin fixed paraffin embedded human angiosarcoma stained with VEGF Receptor 1, using ABC and AEC chromogen.
