Protein A/G Sepharose® Column (ab193263)
Overview
-
Product name
Protein A/G Sepharose® Column -
Product overview
Contents:
Supplied as ready-to-use pre-packed columns of 1 mL or 5 mL bead volume in PBS with 0.02% sodium azide.
Features:
Binding capacity of human IgG greater than 10 mg/mL of bead; high flow rate; low falling off of rProtein A/G; pH stability 2-10.
Store column at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
Purification of monoclonal and polyclonal antibodies.
Sepharose is a registered trademark of GE Healthcare
-
Notes
Protein A/G Sepharose® is prepared by covalently coupling recombinant Protein A/G (contains five Ig-binding regions of protein A and three Ig-binding regions of protein G) to 6% cross-linked Sepharose® beads. The coupling was optimized to give a high binding capacity for IgG. The capacity of IgG binding could be greater than 10 mg of human IgG per ml of wet bead.
-
Tested applications
Suitable for: Purificationmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 5 ml Protein A/G Sepharose® Column 1 x 1ml 1 x 5ml -
Research areas