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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class II

Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (ab248398)

Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (ab248398)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7982] to HLA DMB - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-HLA DMB antibody [EPR7982] - BSA and Azide free
    See all HLA DMB primary antibodies
  • Description

    Rabbit monoclonal [EPR7982] to HLA DMB - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon tissue and tonsil tissue. ICC/IF: Raji cells and 293T cells. WB: HEK-293T cells transfected with a human HLA-DMB expression vector containing a his tag whole cell lysate, Raji, and Daudi cell lysates.
  • General notes

    ab248398 is the carrier-free version of ab131273.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7982
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class II

Images

  • Western blot - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (ab248398)
    Western blot - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (ab248398)
    All lanes : Anti-HLA DMB antibody [EPR7982] (ab131273) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Daudi cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 29 kDa



    This data was developed using ab131273, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (ab248398)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (ab248398)
    This data was developed using ab131273, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling HLA DMB with ab131273 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (ab248398)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (ab248398)
    This data was developed using ab131273, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling HLA DMB with ab131273 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (ab248398)
    Immunocytochemistry - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (ab248398)
    This data was developed using ab131273, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of Raji cells labelling HLA DMB with ab131273 at 1/250 dilution.
  • Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (ab248398)
    Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (ab248398)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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